【摘要】 应用人血在体外培养,分别用PWM和LPS诱导淋巴细胞,接受各种剂量⁶⁰Coγ线照射,两种细胞按各种组合混合再培养,并设有各种单项培养作对照,以³H-TdR掺入反映淋巴细胞的增殖转化。实验结果说明PWM细胞能够激活LPS细胞转化,当一种细胞受照后单独培养或与另一种正常细胞混合培养,其放射性掺入即下降,并随剂量增加而愈益明显、下降趋势均呈现负相关,并分别得到直线回归方程,差异显著性比较说明PWM细胞受照影响更严重。当PWM细胞接受1Gy以上剂量后与正常LPS细胞混合培养即失去激活作用,LPS细胞受照1—2 Gy仍然可被激活,以上提示电离辐射导致机体免疫缺陷,其中PWM诱导的T辅助细胞受损是较重要的。更多还原

【Abstract】 Human whole blood were incubated in vitro. Lymphocytes were in-duced by mitogen PWM and LPS respectively. After exposure to ⁶⁰Coγ-rays at variousdoses, the cells were incubated together in different combination. Lymphocyte inducedby one kind of mitogen was incubated as control. The rate of lymphocyte pro1iferationand transformation was determinated by ³H-TdR incorporation. This study demonstra-ted that PWM-induced lymphocyte was able to enhance the stimulating effect of LPSon B lymphocyte. Lymphocytes induced by one kind of mitogen were exposed to 1-40Gy r-rays, and the cells were incubated alone or with the lymphocyte induced byanother mitogen, thus, the ³H-TdR incorporation became much lower than that in con-trol. The result showed that the decrease in incorporation of ³H-TdR was dose depen-dent and it had a negative correlation. The relationship between the logarithm of ra-diation dosage and the radioactivity counts for ³H-TdR incorporation was expressedby the linear regression equations. By T-test, it was shown that PWM-induced lym-phocyte was damaged more markedly than LPS-induced lymphocyte by irradiation. Thestimulating effect of the PWM-induced lymphocyte disappeared after 1 Gy irradiation.but the LPS-induced lymphocyte after 1-2 Gy irradiation was still stimulated by thePWM lymphocyte. It demonstrated ionizing radiation caused defect of immunity inorganism, the damage of the PWM-induced T helper lymphocyte was more importantin maintaining the function of immunity.更多还原