【摘要】 伊贝母鳞茎培养在附加2,4—D、IAA、NAA和2,4—D+KT、IAA+KT、NAA+KT的MS培养基上(2,4—D、IAA、NAA1毫克/升,KT0.1毫克/升),研究了愈伤组织细胞染色体的变异及愈伤组织的分化和再生植株的染色体倍性。结果表明,2,4—D能有效地引起染色体数目的变化,当和KT结合使用时,可诱导高频率的多倍化细胞。IAA的作用次之,NAA较小。各种激素均能程度不同地引起各种类型的有丝分裂异常及染色体结构变异,其效应与对染色体数目变异的影响呈现明显的一致性。研究还得出,染色体的整倍性是愈伤组织得以分化的重要因素,所以再生植株主要是二倍体,也有少量的四倍体,混倍体仅占少数。根据实验结果,对染色体变异的原因以及染色体数目变异与愈伤组织分化的关系进行了讨论。更多还原

【Abstract】 The cutting pieces of bulb of Fritillariae pallidiflorae were cultured on MS media supplemented with 2, 4-D, IAA, NAA or 2, 4-D+KT、 IaA+KT, NAA+KT. (2, 4-D, IAA, NAA 1mg/l, KT 0.1mg/l). Chromsome variations in calli and callus differentiation and chromosome ploidy of regenerated plants were studed. The results showed that 2, 4-D could give rise clear changes of chromosome numbers. A higher frepuency of polyploid cells appeared on the medium with 2, 4-D+KT, and then IAA、 NAA only produced little effect. However, all of these hormone combinations could cause different types of mitotic abnomality and the variations of chromosome structure. Such effects quite corresponded with that on variations of chromosome numbers. Me Study also showed that chromosome euploidy was important factor for callus differentiation. Therefore, most of the regenerated plants were diploid, a few of them were tetraploid, the mixoploids were seldom seen. In accordance with results of our experiments, the possible reason for chromosome variation and relations between variation of chromosome numbers and callus differentiation were discussed.更多还原