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应用ELISA检测肺吸虫病人血清中特异性IgG、IgM和IgA

Detection of Serum IgG, IgM and IgA with ELISA for Paragonimiasis

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【作者】 张月清侯林浦温艳桂淑环许炽熛

【Author】 Zhang Yueqing, et al

【机构】 北京热带医学研究所北京中日友好医院检验科北京热带医学研究所

【摘要】 用ELISA法对91例肺吸虫患者血清检测IgG、IgM和IgA抗体,结果IgG全部为阳性,OD均值为1.08;IgM 35例(38.5%)阳性,OD均值为o.39;IgA全部为阴性。对30例进行阳性终点滴定,IgG和IgM最高滴度分别达1:102 400和1:1 600。对17例肺吸虫患者治疗前和治疗后半年至3年取血检查,IgG的OD值均有所下降,其中6例已转为阴性。IgM在治疗前8例阳性,治疗后全部降至正常水平。

【Abstract】 The sera of 91 cases of Paragonimiasis were examined for lgG, lgM, and lgA by the ELISA method. The results revealed the presence of lgG antibody in all serum specimens (mean OD value 1.08). lgM was present in 35cases(38.5%);IgA was absent in all cases.Thirty serum specimens were diluted to their terminal point. The highest titre for lgG was 1:102,400;for lgM,it was 1:1 600.Follow-up examinations were conducted on 17 patients at six months and three years after bithionol treatment. The examination showed a reduction of OD value of sera lgG; six becamer negative. Eight of the 17 cases in which the OD value of the serum lgM was positive before treatment had normal levels of lgM on followup examination.

【关键词】 IgGELISA检测IgAIgM肺吸虫病吸虫滴度阳性例数高度敏感性结合物
  • 【文献出处】 北京医学 ,Beijing Medical Journal , 编辑部邮箱 ,1987年04期
  • 【下载频次】51
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