【摘要】 用乳腺癌患者的原发瘤组织制备的细胞膜抗原免疫BALB/C小鼠,取致敏脾脏细胞与SP_2/0骨髓瘤细胞融合。融合率为42％,经克隆化培养后得到一株分泌抗人体乳腺癌单克隆抗体杂交瘤细胞,命名为6C₆。其分泌的抗体和乳腺癌细胞系MCF-7呈阳性反应,与其它5种人癌细胞系,1种人正常细胞系及4种鼠类肿瘤细胞系呈阴性反应。免疫组织化学实验表明:6C₆单克隆抗体（简称6C₆McAb）与乳腺癌的反应多呈阳性（30/36）,与7例正常乳腺均呈阴性反应。6C₆McAb类别为IgC₁。其抗体经ZetaPrep大容量离子交换圆盘纯化,对乳腺癌原发瘤组织仍具有较高的活性。更多还原

【Abstract】 6C6 Monoclonal antibody was produced from a fusion of spleen cells to plasma membrane of a human breast cancer-primed BALB/C mouse with the murine myloma cell line SP2/0. Positive 6C6 hypridomas were single cell cloned. The antibody reacted strongly with the plasma membrane of human breast cancer cells MCF-7. Tissue section of both human mammary carcinomas and benign breast lesion and tumor of non-breast origin as well as apparently normal tissue were tested with immunoperoxidase. 30/36 carcinomas of breast reacted positively. 0/7 human normal mammar tissue reacted negative. Little or no appreciable reactivity was observed with benign breast lesion and carcinoma of non-breast origin. Subclasses of 6C6 Monoclonal antibody was更多还原