【摘要】 本文采用琼脂糖4B交联不溶性丙酮酸类似物（草酸盐衍生物）的亲和层析法及DEAE-Sephadex A50层析柱两种方法对北京鸭心肌LDH₁进行分离纯化比较。 琼脂糖4B交联丙酮酸类似物层析柱具有高的亲和特性。比较经过亲和前后的样品通过DEAE-Sephadex A50柱二次纯化后,发现LDH同工酶在聚丙烯酰胺凝胶电泳图谱上出现二条具LDH酶活性的蛋白条带。其中LDH₁的量远远超过LDH₂的量。 在等电聚焦电泳的实验中,发现多肽现象电泳图谱表明LDH₁有10条亚带。LDH₂有9条亚带。更多还原

【Abstract】 We separated heart LDH, of Peking Duck by Sepharose-4B-CL-Oxamate affinity chromatography column and by DEAE-Sephadex A50 column.The Sepharose-4B-CL-Oxamate column has high affinity with LDH isoengyme. Eluant with an increasing solt gradient to 0.085M NaCl on DEAE-Sephadex A50 column. Two active bands of LDH isoengyme LDH1 and LDH2 were separated, while LDH1 was of more amount than that or LDH2. Protein pattern was obtained on PAGE plate. They are purified by Sepharose-4B-CL-Oxamate or by DEAE-Sephadex A50 column, or both. It was also found in the IEF test, that LDH1 Consested 10 subbands and. LDH2 of 9 subbands of polypeptiche pattern.更多还原