【摘要】 本文用脊髓灰质炎病毒3个型6个强弱代表株壳蛋白一级结构,借助电子计算机预测和计算出病毒壳蛋白的二级结构和亲水概率。更多还原

【Abstract】 Results o.f microcomputer analysis are reported on the prediction and comparison of secondary structures and surface probabilities of the ca.psid proteins of 6 reference strains of the three type of polioviruses and on the secondary structure basis for antigenic sites on the surface of capsid and for neurovirulent attenuation. The results showed: ( 1 ) there are some similarities in the secondary structure of VP1, VP2 and VP3 with 8 or more sheets in each of the 3 proteins, ( 2 ) the 5 peptides capable of priming immune system in rabbit for an IgG response are formed by characteristic turn structures and it is speculated that only a turn can be an antigenic determinant and the increase, decrease, translocation of turn or residue substitution in the turn may be the structural basis for antigenic change of poliovi-rus; ( 3 ) attenuation does not basically cause changes of secondary struc-turse of viral proteins, but cause changes of hydrophilic surface probabilities of some regions of the viral proteins; (4) according to surface probabilities, intratypic-specific regions and intertypic specific regions are identified. These results are of importance in designing synthetic vaccine and re-combinant vaccine against poliomyelitis.更多还原