【摘要】 <正> 本文报道新城疫Ⅰ系和Ⅳ系疫苗病毒的分离纯化,以及对其结构蛋白的初步鉴定。 将中国兽医药品监察所提供的冻干苗(NDV Ⅰ系和Ⅳ系)复壮2次,使其鸡胚感染滴度达10~6,血凝滴度为640(0.1ml)以上。按常规接种9～10日龄受精SPF鸡胚,35℃培养,收集36～72小时鸡胚尿囊液,测定血凝及滴度,置-20℃冰箱备用。更多还原

【Abstract】 Two vaccinal strains of Newcastle Disease Virus ( NDV ) were inoculated separately in the allantios cavity of 9 or 10 day-old Specific Pathogen Free (SPE) fertile eggs.The eggs were incubated for 36-72hr before harvesting the allantoic fluid. The virus was purified with a modification of the method by Meager. Infected allantoic fluid was concentrated by differential precipitation and linear gradient centrifugation in 20-60% sodium potassium tartrate for 14-18hr. The particles of the purified NDV under electron microscope showed a homogenous population of roughly spherical particles covered by spikes on their surface. Antigencity was determinated by double diffusion. Characterization of the structural polypeptides were examined on the SDS-PAGE gels No difference was seen in the structural polypeptides of the two NDV vaccinal strains (Ⅰ and Ⅳ line).更多还原