【摘要】 <正> Kasamo等人曾测定了烟草花叶病毒感染的烟草叶肉细胞质膜上三磷酸腺苷(ATP)酶的活力,它高于对照者2～4倍。他们认为烟草叶肉细胞质膜ATP酶活性的上升与烟草花叶病毒的侵染有关。近十余年来,应用原生质体为材料,研究植物病毒获得一些有意义的结果。本文报导青菜原生质体的制备及其ATP酶的特性,并初步研究了长叶车前花叶病毒更多还原

【Abstract】 The paper reports the property of adenosine triphosphatase of the whole cell protoplast of Brassia chinensis. The enzyme activity changed during early stage after infection by the Shanghai isolate of Holmes’ ribgrass virus. The ATPase activity of the protoplast was activated by cation ( Co++≥Mg++ ≥Ca++≥K+ ≥Ni++≥Mn++≥Fe++) . For Ca++ -activation of the ATPase at ca. 38℃, the pH optimum was nearly 6.0, the apparent Km value of ATPase was 5×10-3M. Forty minutes after virus infection the ATPase was increased.更多还原