【摘要】 长叶车前花叶病毒上海分离株（HRVsh）的外壳蛋白中含有4个甲硫氨酸残基,本文采用溴化氰裂解,并结合葡聚糖凝胶G-100柱层析、高压纸电泳及纸层析等方法,分离纯化了5个多肽片段,经¹²⁵I标记抗体对免疫多肽的鉴定,表明其中二段多肽与¹²⁵-IgG的结合能力接近完整病毒的水平,说明这二段多肽具有HRVsh的抗原专一性,决定HRVsh抗原性的抗原决定簇主要分布于这二个肽段中。 外壳蛋白的胰蛋白酶酶解肽谱及多肽氨基酸序列分析的结果,表明HRVsh和HRV标准株系间在氨基酸序列上有很大相似性,这就决定了两者密切的血清学亲缘关系。更多还原

【Abstract】 It has been shown previously that there are four methionine residues in the coat protein of HRVsh. In this paper, the coat protein was divided into five peptide fragments by chemical cleavage of methionyl bonds with cyanogen bromide.Each fragment was separated and purified by gel filtration on Sephadex G-100, paper chromatography or high-voltage paper electrophoresis. Two peptides were found to possess specific immunological activity related to the whole protein, because they showed immunological reaction with the 125I-IgG against the HRVsh. This fact indicates that the antigenic determinants in HRVsh protein may be distributed in these two immuno-peptides.Peptide mapping of the tryptic peptides of HRVsh protein and amino acid sequence analyses of N-terminal and C-terminal of the two peptides revealed quite similar amino acid sequence between HRVsh and HRV, paralleling the close immunological relationship between HRVsh and HRV.更多还原