【摘要】 本文叙述了影响日本着色系富士“长富2”休眠条催芽及其离体繁殖和生根因子。经过冷冻处理的休眠条浸在BA(1mg/l)溶液中可产生最多的新枝条。首次接种的芽在有BA1mg/l和IBA0.1mg/l的LS培养基生长良好。MS比LS培养基更有利于微枝条的繁殖,其繁殖系数在一定范围内随培养时间增加而增加。生根以在含有NAA(0.25mg/l)和IBA(0.25mg/l)的混合培养基中为最好,有70%生根。(缩写词:LS,linsmain and Skoog;MS,murashige and Skoog;BA,6—苄基氨基腺嘌呤;NAA,萘乙酸;IBA,吲哚丁酸)。更多还原

【Abstract】 This paper describs the factors affecting the sprouting of the dormant sho-ots used for tissue culture, their shoot proliferation and root initiation of theapple"Coloured Fuji No. 2" in vitro. The best forcing solution for cold treateddormant shoots was the water with BA at I mg/1. The single node explantsestablished very well in LS medium with BA at I mg/1 and IBA at 0. 1mg/1.For microshoot proliferation,MS was better than LS medium. The proliferationcoefficient increased with the time of explants development. The NAA- containingmedium gave more roots than IBA- containing medium. LS medium withNAA at 0.25mg/1 and IBA at 0.25mg/1 gave the best root initiatin (70%rooting).更多还原