【摘要】 <正> 我们试图研制人体小细胞肺癌单克隆抗体作为诊断、治疗中一个新途径的探索,同时提供研究小细胞肺癌抗原研究的工具。本文报道利用人体小细胞肺癌细胞株为免疫原,研制的杂交瘤单克隆抗体2F₇、4B₈和6H₁₀的初步结果,在ELISA法和对细胞悬液滴片及组织切片的ABC染色法中,显示这更多还原

【Abstract】 Mouse myeloma cells were fused with spleen cells from BALB/C mice immunized withcell line of human small cell lung carcinoma. The hybrids were screened for monoclonalantibody production with intact cells in ELISA test and ABC staining. Three monoclonalantibodies SCI-2F7, 4B3 and 6H10 were chosen. In the ELISA binding assay, 24 celllines including liver cancer, stomach cancer, breast cancer, various leukemia and normallung were tested. The antibodies only reacted with the cell lines of small cell lung cancerneither with normal cell nor with other tumor cells. In the cell suspension ABC staining testthe antibodies were found reacted with some lung adenocarcinoma cell or liver cancer cellsweakly. Using immunobloting test, the M. W. of these antigen 2F7 and 4B3 are 50KDand 42KD respectively. We also observed a special reaction band appearing on 2F7 and4B3 with small cell lung carcinoma cells. The results suggest that further study is neces-sary to make clinical application of the antibodies possible.更多还原