【摘要】 <正> 近年来的研究提示,癌变时癌基因被激活,这些癌基因相应的蛋白产物在癌变中有一定生理功能。我们曾显示了N-ras基因是人体肝癌转化基因之一;用人体原发性肝癌及肝癌细胞株7402的DNA所转染(transfection)的NIH/3T3细胞中的DNA有人的N-ras基因,而且这种经转化的细胞及7402细胞中N-ras的产物——分子量为更多还原

【Abstract】 Using the NIH/3T3 cell line in which the DNA was transfected with human livercancer and in which the presence of human N-ras oncogene and P21 over expression hadbeen demonstrated, BALB/C mice were immunized. The spleen cells from these immunizedmice were fused with the myeloma cells of SP2/0 mouse. Selected in HAT medium andscreened with ELISA technique, the hybrids were cloned for 5 times, and two clonesOncogema 1 and 2 have been established as cell lines. According to the immunoprecipitationtest,the molecular weight of protein reacted with Oncogema-I was 21000. In further study,this protein with M. W. 21000 has been demonstruted to possess a binding capability toGTP, i. e. the unique charactor of ras oncogene product P21. Testing the reactivity ofOncogema-I with 22 various human malignant tumor cell lines and normal cells, we foundthe liver cancer cell lines Q₃ and 7402 strongly reacted with the monoclonal antibody ofOncogema-I besides the transfected NIH/3T3 cell line. Using ABC staining, it was foundthat P21 was much more concentrated in the liver cancer cells than in the normal cells, inliver cancer specimens test.更多还原