【摘要】 本文报道用含有人羊膜细胞系的各种培养系统,培养周期型马来丝虫感染期幼虫（L₃）,在添加10％小牛血清、葡萄糖的RPMI1640培养液中,L₃发育至Ⅳ期蚴（L₄）,存活40天以上。实验结果证实了葡萄糖对体外培养的周期型马来丝虫L₃生长、发育和存活有促进作用。更多还原

【Abstract】 In vitro culture system supplemented with mammalian cell lines were feted for their ability to sopport the development of Brugia malayi infective larvae to the fourth or fifth stage. This paper reports that the infective larvae of peridic Brugia malayi develop to the fourth stage and survive for excess of 40 days in cultare media RPMI1640 sapplemented with 10％ new born calf serum, glucose and human amnion cell line(FL) as a feeder layer. The study has demonstrated that glucose can facilitate growth, development and survival of the infective larvae of peridic Brugia malayi.更多还原