【摘要】 通过电镜和¹²⁵Ⅰ—udR标记肿瘤靶细胞DNA释放试验,对腹腔或皮下注射SpA或SpA菌体后小鼠腹腔巨噬细胞的超微结构、及其对靶细胞的细胞毒效应进行了动态观察。结果首次证明,小鼠腹腔注射SpA菌体后第5、14d,其腹腔巨噬细胞体积明显增大,可达对照组的2—3倍;皱褶明显增多,有众多的伪足和指状突起,外形极不规则,形似海星;胞质内细胞器明显增多,尤以粗面内质网和溶酶体为显著,胞质内充满吞噬体。实验进一步证明,小鼠腹腔注射SpA或SpA菌体后第5d,其腹腔巨噬细胞杀伤效应（细胞毒分别为42.0±7.8％和46.3±11.7％）比对照组（细胞毒为32.0±13.8％）明显增强,至第7d,细胞毒效应达峰值水平（分别为50.4±17.3％和56.9±13.4％）。根据实验结果,对SpA增强巨噬细胞杀伤效应的可能机理进行了讨论。更多还原

【Abstract】 After injecting the mice with staphylococcal protein A（SpA） or ApS containing staphylococci via different routs, i. e, subcutaneous or intraperitoneal, dynamic changes of ultrastructure and cytotoxic effect of maeorophages in the intraperitoneal cavity were observed by the electron microscope and the release test of ¹²⁵Ⅰ-udR labeled tumor target Cell DNA. It was fount that, 5-14 days after injecting the mice with SpA containing staphylococci intraperitoneally, volume of the murine peritoneal cavity macrophages was augmented remarkably, being 2-3 times as large as that of the control group. The surface folds of macrophages were significantly increased in comparison with control group. SpA containing staphylococci-activated macrophages were very irregular, with many pseudopodiums and finger-like process, starfishlike in shape. There was marked enhancement in cell organs-especilly, rough endoplasmic retieulars and lysosomes and there was abundant phagosomes in the cytoplasm. Furthermoren, the results showed that, 5 days after injecting SpA or SpA containing staphylococci into the mice intraperitoneally, cytotoxic effect of the mae rophages in the peritoneal cavity was increased more significantly than that of the control group（cytotoxicity 32.0±13.8％）, reaching 42.0±7.8％ and 46.3±11.7％. The cytotoxic effect reached peak levels（50.4±17.3％ and 56.9±13.4％ respectively）on 7 the day. Based on the experimental results, possible mechanism in the cytotoxic effect of macrophage increased by SpA was diseeused.更多还原