【摘要】 鸭HDL-apo CⅡ不含唾液酸;按SDS-PAGE测定,其分子量为10058 d;氨基酸组成分析显示其含18种氨基酸,没有半胱氨酸和组氨酸。因此鸭HDI-apo CⅡ为一单链多肽。在等电聚焦电泳中,鸭HDL-apo CⅡ显示三种多态型,其中主带的等电点为5.07,两次带的等电点分别为4.87和4.84。更多还原

【Abstract】 Among apolipoprotein C subclasses, aPo CⅡ is one of the most widely investigated. Since 1970, papers on structure and function of apo CⅡ and its relationship to plasma lipid and hyperlipoproteinemia have been re- ported. In another paper, we have reported the isolation and characterization of duck HDL-apo CⅡ. In order to get more information about duck HDL-apo CⅡ, we determined its molecular weight, isoelectric points and sialic acid, polymorphisms and amino acid composition.Duck HDL-apo CⅡ does not contain sialic acid. Its molecular weight is 10058 daltons on SDS PAGE. According to analysis of amino acid composition, it is composed of 18 kinds of amino acids but not Cys-SH or His, implying duck HDL-apo CⅡ2 being a single chain polypeptide. In the meantime, it contains high content of polar amino acids, especially basic amino acid which will facilitate the formation of amphoteric helix.HDL apo CⅡ shows three polymorphisms in PAGIFE in which the pI of major band is 5.07 and that of two minor bands are 4.87 and 4.84 respectively.更多还原