【摘要】 本文研究了含丰富HDL的雄性北京鸭,对胆固醇(Ch)的代谢特点及其与抗动脉粥样硬化(AT)的关系。在18周饲养高Ch和高脂肪饲料期间,实验组(16只)血清Ch和HDL-Ch与对照组(14只)比在8周后均有明显增加,分别平均从132±20mg/dl最高增加到158±28mg/dl,从81±15mg/dl最高增加到115±9mg/dl,血请βLP仅在8周时略有增加,α和βLP％量则基本不变,甘油三脂和LCAT酶活性二组间无显著差异,PAG电泳显示蛋白染色二组无明显差异,脂质染色HDL带变宽,说明北京鸭主要由血清HDL运转外源性Ch,此外,二组主动咏均无粥样硬化斑块形成,仅有脂肪条纹。本实验为以HDL作为运转Ch者的动物有抗AT作用提供了有力的实验依据。更多还原

【Abstract】 Previously we have found Beijing duck, similar to tree shrews, had a high content of high density lipoprotein. The present study is to see whether Beijing duck would also be difficult to develop atheromatous plaque as tree shrews under a high cholesterol and high fat diet.Thirty male ducks of 3 years old with average body weight of 2.5 kg. were fed on a high cholesterol and high fat diet for 18 weeks. Before experiment, the serum cholesterol, HDL-cholesterol, VLDL + LDL level, α-and β-lipoprotein percentages, triglyceride, LCAT activity and PAGE of Beijing duck were determined, the values being 134±35mg/dl, 82±25mg/dl, 76.8±6.0% and 23.2±5.7%, 97±40mg/dl and 82±30 nmol/h/ml respectively. The ducks were divided into two groups:the control (14n) and the experimental (16n), according to the level of serum cholesterol. During experiment, the above items were assayed once about every 4 weeks. At the end of 18 weeks, the aortae were examined with both light and electron microscopeso following results were obtained:During 18 weeks of experiments serum total cholesterol and HDL-cholesterolincreased significantly, with the highest values of 158±28mg/dl and 115±9mg/ dlrespectively while only a slight rise was observed in VLDL + LDL. Moreover,a wider band of HDL was found in PAGE in the experimental group than thatof the controls. However, the rest of the items assayed remained practically the same as the controls, indicating that most of the cholesterol fed was mainly carried by HDL rather than LDL as in man and in rabbit. Furthermore, no atheromatous plaque was observed in both the experimental and control groups, only fat precipitations in the intima were found. Thus, another experimental evidence was found by us showing that HDL may have the anti-atherogenesis effect in other species besides tree shrews. The different characteristics of transporting cholesterol by serum lipoproteins in different animals and their role in the anti-atherogenesis are discussed and a hypothesis was put out.更多还原