【摘要】 利用组织培养和系统选育,从杂交水稻"南优2号"、"汕优2号"中获得的新品系"南花5号"、"南花11号"、"汕二39"、"汕二59"是高产品系,用淀粉凝胶和聚丙烯酰胺凝胶电泳分析了这些新品系及"南优2号"、"汕优2号"杂交种和亲本中的酯酶、过氧化物酶、ATP酶、苹果酸脱氢酶、谷氨酸脱氢酶的同工酶。杂种"南优2号"和"汕优2号"及其亲本之间的ATP酶、苹果酸脱氢酶、谷氨酸脱氢酶的同工酶都没有差异。杂种"南优2号"、"汕优2号"酯酶同工酶酶谱、阳极有EA₂、EA₃互补酶带,阴极有Ec₁、EC₂、EC₃、EC₄互补酶带,"南花5号"、"南花11号"、"汕二39"、"汕二59"的酯酶同工酶酶谱型能从F₂代的杂交稻中选育得到。表明通过组织培养或系统选育或结合理化因素处理都能从优良杂交稻的分离代中获得这些高产新品系。更多还原

【Abstract】 The new lines Nanhua 5, Nanhua 11, Shanyou 39, and Shanyou 59, are high-yield lines derived from the F₁ hybrid rice, Nanyou 2 and Shanyou 2 by means of tissue culture and selection. Five isozymes, esterase, peroxidase, ATP-ase, malate dehydrogenase and glulamate dehydrogenase from the new lines, the F₁ hybrid rices and their parents, were analyzed by starch and polyacrylamid gel electrophoresis. There are no difference in zymogrames of ATPase, malate dehydrogenase, glulamate dehydrogenase, between the F₁ hybrid and its parents. But the F₁ hybrid rice contains complemental bands of anodal esterase EA₂, EA₃ and cathodal esterase EC₁, EC₂, EC₃, EC₄, The esterase zymogram of seeds of Nanhua 5, Nanhua 11 and Shanyou 39, Shanyou 59 could be observed in the progeny of the F₂ hybrid rice. The results indicated that the new lines would be possibly derived from the progeny of the F₁ hybrid by means of tissue culture. and selection.更多还原