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叶绿体膜发育不完善的小麦的荧光
FLUORESCENCE OF WHEAT WITH INCOMPLETELY DEVELOPED CHLOROPLAST MEMBRANES
【摘要】 叶绿体膜发育不完善的小麦的叶绿体低温(77K)荧光发射光谱十分类似于大麦突变种,它的短波和长波的发射峰分别在685nm和725nm,表明它的PSI只有内侧天线,而缺乏外周天线。它的叶片的室温荧光诱导瞬变的时间进程与突变种大麦也十分相似,不呈现正常发育的小麦和大麦的典型的O→P→S→M→T振荡的时间进程,而在P峰后只具有单调的衰变,不出现第二峰(M)。荧光诱导动力学的快速荧光上升(O→P)部分,不呈现正常发育的小麦所具有的O→I→D→P的时间进程。
【Abstract】 Low temperature (77K) fluorseence emission spectrum of the incompletely developed ehloroplast membranes of wheat and the fluorescence induction transient of its intact leaf at room temperature were studied. The main peaks of the fluorescence emission spectrum of the incompletely developed ehloroplast membranes were at 685 nm and 725 nm respectively. The positions of these two peaks were almost the same as that in chlorophyll b-less mutant barley Chlorina f2. This showed that the incompletely developed ehloroplast membranes of wheat did not develope peripheral antenna of PSI but only contained internal antenna of PSI as the case of Chlorina f2. The fluorescence induction transient of wheat leaf with incompletely developed ehloroplast membranes did not show the typical time course of O→P→S→M→T and lacked second peak (M) and showed a slow decline as P decayed. This is the same as that of Chlorina f2 leaf. The fluorescence rise in induction period of the leaf with incompletely developed ehloroplast membranes was much different from that of normal wheat leaf. These results can be explained by our previous assumptiont hat the occurence of typical fluorescence induction transient is based on the coexistence of LHCP of PSⅡ and peripheral antenna of PSⅠ and on their cooperation with each other.
【Key words】 Fluorescence emission spectrum; Fluorescence induction transient; PSI; PSII; LHCP; Peripheral and internal antenna of PSI;
- 【文献出处】 Journal of Integrative Plant Biology ,植物学报(英文版) , 编辑部邮箱 ,1986年01期
- 【被引频次】2
- 【下载频次】19