【摘要】 应用正交表L₁₈（6¹×3⁶）对KNO₃、NH₄NO₃、KH₂PO₄、MgSO₄·7H₂O、CaCl₂·2H₂O、铁盐和微量元素的不同水平进行试验,筛选出C₁₇培养基。在7个微量元素试验中,其结果是去掉NaMoO₄·2H₂O和将MnSO₄·4H₂O降为11.2毫克/升较好。 5种有机物中以D-生物素（维生素H）对花药愈伤组织的诱导频率影响最大,以1.5毫克/升最好。1980年河北省农科院粮油作物研究所C₁₇培养基的出愈率达12.19%。花药愈伤组织的质量好,分化率最高达50%。更多还原

【Abstract】 Media with varied levels of minor elements and KNO₃,NH₄NO₃, KH₂PO₄,MgSO₄·7H₂O,CaCl₂·2H₂O and Fe-Salts were screened to obtain high yield of calllus from wheat anther hy using orthogonal tests.Of all the seven minor elements treated,the one without NaMoO₄·2H₂O and with 11.2 mg/1 MnSO₄·4H₂O was found to be the most effective.Among the five kinds of organic materials tested,biotin appeared to have the highest influence on the induction frequency of anther callus and its optimum dosage was 1.5 mg/1.Based on these data,C₁₇ medium was developed and its induction frequency of callus with good quality from wheat anther reached 12.19% in the Institute in 1980.The maximum diffrentiation freguency of anther callus obtained was 50% on C₁₇ medium. C₁₇ medium compositions are as follows （Mg/1） :1400 KNO₃,150 GaCl₂·2H₂O,150 MgSO₄·7H₂O,300 NH₄NO₃,400 KH₂PO₄,27.85 FeSO₄·7H₂O,37,25 Na₂-EDTA,11.2 MnSO₄·4H₂O,8.6 ZnSO₄·7H₂O,6.2 H₃BO₃,0.83 KI,0.025 CuSO₄·5H₂O,0.025 CaCl₂· 6H₂O,8 glycine,0.5 nicotinic acid,0.5 thiamine hydrochloride and 1.5 biotin.The redifferentiation medium is supplemented with 2 2,4—D+0.5 KT+7000 agar + 90000 sucrose, pH 5.8.The dedifferentiation supplemented with 0.5 IBA+2 KT+7000 agar+ 30000 sucrose,pH 5.8.更多还原