【摘要】 <正> 霍乱是一种严重危害人民健康的肠道传染病,发病急,传播快。长期来,人们对本病的控制作了大量工作。但迄今用于霍乱特异性诊断的血清和预防用菌苗均为多克隆抗体和复杂的菌体抗原,难以对菌体抗原成分做进一步分更多还原

【Abstract】 Four strains of monoclonal antibody （McAb） secreting hybridoma cell were established through fusion of mouse myeloma cell and BALB/C mouse splenic cell immunized with Ogawa strain （18001） of Vibrio cholerae （biotype El Tor）.These cells,no matter when they were cultured in vitro or in vivo,were shown to be able to produce McAb against Ogawa,Inaba and Hikojima strains of V.cholerae,with high specificity.The immu- noglobulin of one cell line belonged to IgG_2b subclass,while those of the other three cell lines,all belonged to IgM. The cells grew well and secreted antibody consistently after 10 months of serial passage and 6 months of preservation in liquid nitrogen. The cholera SPA diagnostic reagent made of McAb （2D1） gave satisfactory result in coagglutination test of 49 strains of V.cholerae and 9 strains of other enteric bacteria.更多还原