【摘要】 用有机溶剂提取胡桃花粉中的甾类化合物,以70％甲醇除掉脂类杂质,然后将提取出来的甾类化合物衍生化,经气相色谱分离,质谱仪鉴定,搞清胡桃花粉中含有雌二醇、谷甾醇等甾类激素;植物雄配子体中存在有动物雌性激素。更多还原

【Abstract】 Pollen of walnut （uglans regia） was first extracted with ether, whereby most of the free steroids were obtained. Then the sterol glycosides and esters in the pollen were extracted by acetone. The acetone extracts obtained were hydrolyzed with 1% H₂SO₄ and then with 10% KOH. After that the hydrolyzate was extracted with n-hexane and ether. All extracts were combined and washed with 70% methanol to remove the impurities, then the extract was evaporated to dry ness under vacuum.3β-hydroxysteroids were precipitated after adding a solution of 2% digitonin in 80% ethanol to the dry extract. The impurities consisting mainly of pigments were removed from the precipitate by centrifugation. The purified sterol compounds were allowed to react with bis-（trimethysilyl）-acetamine （BAS） and trimethylchlorosilicane （TMCS） which converted the sterols into their respective trimethylsilyl derivatives. The derived sterols were analysed by GC-MS.The results of analysis showed that pollen of walnut contained estridiol and stigmasterol.更多还原