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梨实生树个体发育过程中蛋白质含量、过氧化物酶和多酚氧化酶同工酶的变化

CHANGES IN PROTEIN CONTENT, PEROXIDASE AND POLYPHENOL OXIDASE ISOZYME PATTERN DURING THE DEVELOPMENT OF PEAR SEEDLING (Pyrus bretschneideri Rehd×P. pyrifolia Burm)

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【作者】 王定祥

【Author】 Wang Ding-xing (Institute of Horticulture, Zhejiang Academy of Agricultural Science)

【机构】 浙江农业大学园艺系 浙江省农业科学院园艺研究所

【摘要】 梨实生树个体童区的童年组织的叶片中,总蛋白和可溶性蛋白含量明显低于成年区的成年组织,过氧化物酶和多酚氧化酶的同工酶谱型也不同子成年组织。而当童年枝条高接在该树的童区和成年区后,主要形态性状、两种蛋白质含量及同工酶谱,分别与非高接的童区、成年区组织有相同表现。高接在童区的成年枝条生长受抑,蛋白质含量下降,但叶片、针刺、节间长度以及同工酶谱型仍表现一定的成年特性。蛋白质含量或同工酶谱的变化与梨实生树组织的成熟有关;个体的发育规律与组织所处的环境有关。

【Abstract】 The total and soluble proteins were greater in adult than those juvenile tissues, in leaves collected from both adult and juvenile zones of seedling trees. The isozyme number and its activity in adult tissue were also greater than those in juvenile. There was significant positive correlation between the total and soluble proteins in leaves and the branch position. The higher the branch position is, the more the total and soluble proteins in leaves increase. Furthermore, after the scions in juvenile and adult tissues were grafted onto the juvenile and adult zones of their own seedling tree, there were significant differences between the scions in adult and juvenile zones. But the leaf size, internode length, thorn and the isozymograms of peroxidase and polyphenol oxidase in the leaves of adult tissue grafted on the juvenile zone still showed certain characteristics of the adult. From these results the author suggested that the appearance of isozymes and proteins may be due to maturation of the leaf tissue. It may be suggested that a series of changes in the expression of genetic information in the tissues of developmental zone were related to the interactions between the environment and the stored genetic information.

【关键词】 梨实生树童性蛋白质同工酶
【Key words】 Pyrus seedlingjuvenilltyproteinisozyme
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