【摘要】 <正> 尿路感染(下称尿感)是最常见的感染性疾病之一,单纯依据临床表现来诊断甚不可靠。然而,许多实验方法都需要作定量培养,且不能立即得出不用培养而接近定量培养准确性的结果,且又无假阴性,为此,国内、外学者均在积极寻求快速诊断尿感的新方法以满足临床需要。最近我们根据Riley等的报道并加以改良,用位相显微镜定量检查不更多还原

【Abstract】 The rapid diagnosis of urinary tract infection remains many problems. More recently TV Riley et al report that phase contrast microscopy examination of the centrifuged deposit of fresh urine could rapidly detect nonsignificant bacteriuria. We have compared phase contrast microscopy (10 × 40) of a noncentrifuged urine in the blood counting chamber of a standard hemocytometer with a dip-slid culture technique for a total of 283 clean-catch midstream urine specimens. Our criterion is that bacteria counted≥10 in a larger unit area was considered a significant bacteriuria; bacteria 3-9 was considered either a significant bacteriuria or a contamination; bacteria ≤2 was considered contamination.40 specinmens dip-slid culture had≥105 colony forming units per milliliter (CFU/ml), of which, 38 specimens examinated by phase contrast microscopy had bacteria 10; 2 specimens had bacteria 3-9. 12 specimens had 104-5 CFU/ml, of which, 6 specimens had bacteria 3-9; 6 specimens had 2. 231 specimens had≤104 CFU/ml, of which, 2 specimens had 10, these 2 cases had kidney stones, urinary tract infections and had taken antimi-crobiol agents; 7 specimens had 3-9. 222 specimens had≤2. Total 283 specimens were examined by the culture and by the phase contrast microscopy, of which, 226 specimens (94%) showed same consequence.These results indicate that the method of examination of bacteria counted in blood counting chamber by phase contrast microscopy serves as a simple rapid correct test to diagnose urinary tract infections.更多还原