【摘要】 <正> 平颏海蛇(Lapemis haidwickii)与青环海蛇(Hydrophis cyanocincius)广泛分布于太平洋、印度洋的-些海域。我国广东、广西、福建、台湾沿海较常见,是我国北部湾产量较多、毒性较大的两种主要海蛇.平颏诲蛇属海蛇科(Hydrophiidae),其毒液含有毒素、酶类及多糖等物质,是供分子生物学研究及临床使用的极贵重药剂.更多还原

【Abstract】 The venom of Lapemis hardwickii,captured at the gulfs of Beihai of Guang-xi province,was collected by the method of Bitten-vessel from living snakeand lyophilized directly.The toxicity was tested by injecting intra-peritoneallythe venom into the mice and had the LD₅₀ value of 0.125mg/kg.The activities of fourteen enzymes from the crude venom were detected.Among them,DNase,Nucleotidase,Acetyl cholinase and Phospholipase A2appeare high activity.No enzymatic activity of L-Amino acid Oxidase and Ar-ginine esterase was detected.It was found that the inhibitor of proteinase exists,the same as in H.cyanocinctus.Four peaks were obtained by running the vonomthrough Sephadex G-75 column.The major lethal peak was further purified bychromatography on CM-Sephadex C-50 for three times.Eleclrophoresis showedonly one band of the final toxin which had 0.037mg/kg of LD₅₀ value.The toxin was determined as a postsynaptic toxin.Our result shows that the method forpreparing the toxin of H.Cyanocintus is also applicable to the purification ofL.Hardwickii’s venom.更多还原