【摘要】 <正> 血红蛋白具有过氧化物酶的活性,它可以催化过氧化氢（H₂O₂）对邻苯二胺（O—pbenylenediamine OPD）等物质的氧化作用,并产生颜色反应,其颜色深浅和Hb含量呈正相关。临床上常用于检测血清中Hb的含量,最近国内外有人根据此原理以红细胞作为靶细胞,通过Hb的释放量检测K细胞的ADCC活性和巨噬细胞(Macrophage更多还原

【Abstract】 An improved assay for quantitation of murine macrophage-mediated antibody-dependent cellular cytotoxicity (MMADCC) by hemoglobin-enzyme release assay (Hb-ERA) has been developed The method is based on the coloration measurement by spectrophotometer,because hemoglobin has peroxidase activity capable of catalyzing the oxidation of o-phenylenediamine and producing color reaction. This method was applied to demonstrate variation of MMADCC activity with varying effector:target ratio,incubation time,antibody concentration and macrophage in different stage of activation. The method provides advantages of(l)elimination of the need for expensive and hazardous radioactive materials,(2)relative ease and rapidity, (3)sensitiyity and reproducibility.更多还原