【摘要】 本文用伊氏牛锥虫小白鼠血片免疫荧光法检测584例血清标本.189例SLE患者用该法检出阳性率达52.3％,24例活动期SLE患者全部呈阳性反应,77例结缔组织病和184例其它疾病及110例健康对照均呈阴性反应. 该法与绿蝇短膜虫免疫荧光法比较符合率达96.3％,并作底物特异性试验,作者认为,本法对活动期系统性红斑狼疮有高度特异性和敏感性,操作简便,结果可靠,重复性好之优点,可作为诊断SLE的新方法.更多还原

【Abstract】 584 human serum samples were tested for anti-ds-DNA antibodies with a new immunofluorescence assay by means of blood smears of mice infected with Trypanosoma evansi as substrate. Results showed a 52.3% positive rate in 189 patients with systemic lupus erythematosus (SLE).All the 24 cases with active SLE showed Positive reactions, but 77 patients with diseases in the connective tissue, 184 patients with miscellaneous diseases and 110 healthy controls gave negative reaction. The result by this method conformed well with that by Crithidin Luciliae assay(96.3%).The authors believe that this technique is highly specific and sensitive in active SLE and is simple, reliable, and reproducible.Thus, it could be applied in the diagnosis and follow-up study on SLE.更多还原