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甜菜胚珠培养诱导单倍体植株的研究

Studies on the Haploid Plant by Ovulture Culture in Sugarbeet

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【作者】 张月琴; 邵明文;

【Author】 Zhang Yueqin Shao Mingwen(The Sugarbeet Research Institute of Academy of Agricultural Sciences of PRC.)

【机构】 中国农业科学院甜菜研究所; 中国农业科学院甜菜研究所;

【摘要】 <正> 植物细胞培养不仅仅局限于体细胞,而且也包括配子体的培养,其中,雄配子体的培养有过大量报导,而关于雌配子体的培养报导甚少。甜菜是我国的重要糖料作物之一,为二年一代的异交作物,通过常规的系谱法和自交手段来获得纯合的材料,不但花费时间长,而且要困难得多,同时还会使许多重要材料被丢失。而通过配子体来获得纯合系,将大大缩短育种年限,并起到提高选择效率的作用。

【Abstract】 The methods of the haploid plant was differentiation from unpollinated ovule of sugarbeet by MS, Nitsh, H media and then obtained the homozy-gous diploid Its chromosomes were doubled by colchicine, all these were reported in this paper. The frequency of induction was up to 35%, 81.4% inoculative material was induced to be seedlings emerged. This inducing method superior to the pollen plant in the range of plant material which can be induced to seedling emerged and inducing frequency etc. This successful method will play the unique role in the sugarbeet breeding.

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