【摘要】 应用自食管癌高发区林县粮食中分离的互隔交链孢霉提取物261-B₂-3诱发人羊膜FL细胞UDS。在不加S-9组分的情况下,提取物可诱发UDS。当提取物浓皮为10^-6-10^-3mg/ml四个数量级时,其UDS诱发率与对照组相比有显著性差异（P<0.05）。且当浓度在2.0×10^-68.0×10^-6mg/ml范阿时,UDS水平与浓度呈正相关且有直线回归关系（P<0.01）。在加S-9组分情况下,各浓度组UDS诱发率均明显低于不加S—9组,与对照相比差别无显著性（P>0.05）。结果表明,在不加S-9情况下,261-B₂-3具有较强的诱变性,其诱变性在一定范围内随浓度增大而升高。相反,在加S-9情况下,261-B₂-3无或仅有极弱的诱变性。提示该霉菌提取物中含有直接诱变性物质。粮食中互隔交链孢霉污染可能在林县食管癌的发生中起一定作用。更多还原

【Abstract】 Tne Unscheduled DNA syntheris （UDS） was induced in cultured human amnionic cell line FL in vitro by exposure to 261-B2-3, an extract of Alternaria alternata separated from crops in Linxian county which is a high risk area of esophageal cancer in China. In the absence of S-9M, 261-B2-3 was capable of inducing UDS in target cells and the ratio of UDS elicited by the extract (the range of concentration being 10^-6 to 10^-3 mg/ml) was significatly different from the ratio by solvent control （P<0.05）. There exist a positive correlation and linear regression between the values of UDS levels and the values of the doses of 261-B2-3 in a range of concentrations (2.0 × 10^-6 to 8.0×10^-3 mg/ml) without S-9M （P<0.01）. The levels of UDS in the presence of S-9M were lower than in the absence of S-9M. There was no obvious difference of the ratio of UDS elicited by 261-B2-3 from that of solvent control （P>0.05） with S-9M. The results demonstrated that 261-B2-3 has stronger mutagenicity without S-9M and it’s mutagenicity is rising with increasing concentrations in a certain range. On the contrary, 261-B2-3 has no or only a weak mutagenicity with S-9M. It is suggested that there arc direct mutagens in this extract. It is possible that Alternaria alternata contaminated the crops play an important role in the high incidence of esophageal cancer in Linxian county.更多还原