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柑桔裂皮类病毒和菊花矮化类病毒互补DNA探针的合成及检测
SYNTHESIS OF CITRUS EXOCORTIS VIROID AND CHRYSANTHEMUM STUNT VIROID DNA 32P-cDNA PROBES AND DETECTION BY THE PROBES
【摘要】 分别用人工合成的柑桔裂皮类病毒和菊花矮化类病毒的互补DNA探针,经固相分子杂交(dot blot)检测了柑桔、菊花等样品。结果表明:与生物学检测和聚丙烯酰胺凝胶电泳(PAGE)分析相比,这是早期诊断类病毒病和检测类病毒的一种较为灵敏、快速的方法。
【Abstract】 The 32P-cDNA Probes of Citrus Exocortis Viroid (CEV) and Chrysanthemum Stunt Viroid (CSV)RNA has been synthesied respectively and usedfor detecting some samples that may contain relative viroids. This is a sensitive and rapid method for diagnosing early viroid disease as compared with biology assays and polyacrylamide gel electrophoresis,
- 【文献出处】 病毒学杂志 ,Virologica Sinica , 编辑部邮箱 ,1986年04期
- 【被引频次】6
- 【下载频次】40