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甲型肝炎病毒分离及培养特性的研究

ISOLATION AND PROPAGATION OF HEPATITIS A VIRUS IN VITRO

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【作者】 李成明陈立礼殷大常陈丹林刘丽华王宏喜胡萍

【Author】 Li Cheng-ming Chen Li-li Ying Da-Chang Chen Dan-lin Lu Li-hua Wang Hong-xi Fu Ping(Department of Viral Disease of Control, Health and Anti-epldemic Station of Sichuan Province, Chengdu)

【机构】 四川省卫生防疫站病毒性疾病控制科四川省卫生防疫站病毒性疾病控制科 成都成都

【摘要】 应用人胚肺二倍体细胞(2BS)直接从甲肝病人急性期粪便中分离培养出一株甲型肝炎病毒(S84=1)。该毒株无致细胞病变作用,具有产量和毒力较高(10-6.5~(-7))繁殖周期短(7天左右)的优点,可作为生产甲型肝炎诊断试剂和研制甲型肝炎疫苗的侯选毒株。

【Abstract】 A Strain of hepatitis A virus(S84-1)was directly isolated from a fecal specimen of acute patient with hepatitis A and serially passaged in human diploid fibroblast(2BS). Some conditions of cultivation of this virus were also studied. Following serial propagation, the incubation time of S84-1 virus was reduced from one and half months(passage 2) to 3 days(passage 12). S84-1 virus, without cytopathie effect, can’t be detected in supernatant fluid by ELISA and was harvested by freezing-throwing the cells 5 times. Comparing with HM-175 HAV strain under the same condition, S84-1 has a higher infective titre(TCID50, 10-6.5—7/ml) and HAAg titre(1: 16, by ELISA ). The typical HAV particles were shown in harvested cell suspension be immune electron microscopy.

  • 【文献出处】 病毒学杂志 ,Virologica Sinica , 编辑部邮箱 ,1986年04期
  • 【被引频次】1
  • 【下载频次】80
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