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甲型肝炎病毒分离及培养特性的研究
ISOLATION AND PROPAGATION OF HEPATITIS A VIRUS IN VITRO
【摘要】 应用人胚肺二倍体细胞(2BS)直接从甲肝病人急性期粪便中分离培养出一株甲型肝炎病毒(S84=1)。该毒株无致细胞病变作用,具有产量和毒力较高(10-6.5~(-7))繁殖周期短(7天左右)的优点,可作为生产甲型肝炎诊断试剂和研制甲型肝炎疫苗的侯选毒株。
【Abstract】 A Strain of hepatitis A virus(S84-1)was directly isolated from a fecal specimen of acute patient with hepatitis A and serially passaged in human diploid fibroblast(2BS). Some conditions of cultivation of this virus were also studied. Following serial propagation, the incubation time of S84-1 virus was reduced from one and half months(passage 2) to 3 days(passage 12). S84-1 virus, without cytopathie effect, can’t be detected in supernatant fluid by ELISA and was harvested by freezing-throwing the cells 5 times. Comparing with HM-175 HAV strain under the same condition, S84-1 has a higher infective titre(TCID50, 10-6.5—7/ml) and HAAg titre(1: 16, by ELISA ). The typical HAV particles were shown in harvested cell suspension be immune electron microscopy.
- 【文献出处】 病毒学杂志 ,Virologica Sinica , 编辑部邮箱 ,1986年04期
- 【被引频次】1
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