【摘要】 黄芪对Coxsackie B-2病毒感染培养的大鼠心肌细胞所致心肌酶-乳酸脱氢酶、谷草转氨酶的释放、搏动停止、细胞病变、细胞毒性及超微结掏改变等均有明显保护作用。尤其在感染后1—9小时给药保护作用完全。给药后病毒滴度仍能测得,但明显低于病毒对照组。本结果可作为进一步防治急性病毒性心肌炎提供线索。更多还原

【Abstract】 The protective effect of Astragalus Membranaceus（AM） on Coxsackie B-2 virus infected newborn rat heart cell cultures was observed. The cardiac enzymes-lactic acid dehydrogenase （LDH） and glutamic oxaloacetic transaminase （SGOT） in infected group were much higher than in those groups of uninfected. AM control, and AM treated 1 hr after inoculation with 100 TCID50 Coxsackie B-2 virus for 2—5 days （p<0.01）. The beating percentage began to decrease in infected group at the 2nd day and-no beating was shown 3—5 days after virus challenge. Meanwhile the CPE appeared rapidly from 1+—3+ at the same interval. In contrast, the beating was 100% and no CPE was shown in the other 3 groups throughout. The cytotoxicity of ⁵¹Cr-labbeled heart cells treated with AM 1 hr after virus challenge was lower than that of infected cultures at 72 hr （p<0.05） and 96 hr （p<0.01） after infection. A significant protective effect was provided when AM was treated among 1—9 hr after virus inoculation. The protection was incomplete if AM was treated 12—18 hr after infection, and no protection was seen if treated with AM 18 hr before or 24—48 hr after virus challenge. Virus tilers detected in the cultures treated with AM 1 hr after infection were much lower than that in the infected cultures—either in cells or in supernatants through 2—5 days, In addition, the virus liter was similar between infected and infected-AM treated cultures in case AM was treated 18 hr before virus inoculation. The ultrastructural alterations of the myocardial cells including mitochondria, intercu lated discs and myofibers also showed nice protective effect in infected-AM treated group through 2—5 days after virus challenge, These results could provide the favourable factors for acute, Coxsackievirus myocarditis prophylaxis and therapy,更多还原