【摘要】 本文建立了13侏乙脑单克隆抗体杂交瘤细胞系,对其中6株进行了特性研究,结果表明该6株中3株为乙脑病毒种特异性抗体,3株为乙脑病毒亚组特异性抗体。以免疫荧光技术对12株国内外分离的乙脑病毒进行了抗原性比较,结果表明我国分离的大多数病毒株间无明显的抗原性差异,但个别近年来分离的病毒侏如云南的Y-M60,福建的NY,湖南的X8103株与1949年北京分离的P₃株即目前用于灭活疫苗生产的病毒株间有一定的差异。我国的大多数病毒株与日本的中山（NaK）株则有明显的差别。此外,并发现2株单克隆抗体对我国的全部野病毒株有高滴度的强荧光反应而对14-2减毒株仅有十分微弱反应,甚至无反应。以上结果提示乙脑不同病毒株间存在抗原性差异,但是否有免疫保护上的抗原性差异尚待进一步研究。更多还原

【Abstract】 Thriteen hybridoma cell lines secreting McAbs against IEV have been established. Six of them were selected for study. The characterization of the six cell lines of McAb indicated that three of them were JEV—species specific and the other three were JEV—subgroup specific. In this paper, 12 JE virus strains, including 10 Chinese strains, one Japanese strain and one attenuated virus, were selected for antigenic analysis with the six cell lines of McAb. The result showed that most of those Chinese strains were antigenically identical, however, the antigenicity of three strains recently isolated from Yunnan, FuSian and Hunan provinces showed considerable difference from P₃ strain, isolated in 1949 and now used for production of JE killed vaccine. The result also revealed that most JE virus strains isolated in China showed markcd difference with Japanese NaK strain. Besides, two cell lines of McAb were found to react with all the 10 Chinese wild JEV strains to a high litre （≥1280） but very low （10—9.0） with 14—2 attenuated virus. The significance of this discrepancy deservc further investigation.更多还原