【摘要】 使用限制性核酸内切酶 BamHI 将油桐尺蠖核型多角体病毒（Buzura SuppressariaNuclear Polyhedrosis Virus。简称 BsNPV）DNA 切为9个片段。通过聚丙烯酰胺凝胶电泳洗脱,将其酶切片段与 BamHI 切割后的质粒 pBR₃₂₂重组,转化大肠杆菌,经抗菌素筛选、电泳分折和菌落杂交3种方法证明了插入子的存在,从而表明油桐尺蠖核型多角体病毒 DNA BamHI 片段已经克隆。更多还原

【Abstract】 The DNA of Buzura Suppressaria Nuclear Polyhedrosis Virus（BsNPV） was digested at least9 fragments by restriction endonuclease BamHI.The fragments eluted by polyacrylamide gel electrophoresis were recombined with plasmid pBR₃₂₂incubated with BamHI and transfered into E.coli. Antibiotic screening,electrophoresis analysis and colony hybridization showed the existence of insert sequence in hybrid plasmid.The result suggested that the cloning of BamHI fragments of BsNPV DNA be successful.更多还原