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气相色谱法测定人体血液中游离脂肪酸
ANALYSIS OF FREE FATTY ACID IN HUMAN BLOOD BY GAS CHROMATOGRAPHY
【摘要】 血液中游离脂肪酸经氯仿抽提后,用BF3-甲醇酯化成脂肪酸甲酯,进行气相色谱测定。内标准采用正十七酸。本方法酯化率为99.4%±1.4,回收率为86.4%±1.9,变异系数(CV)为6.9%。
【Abstract】 Serum free fatty acids were isolated with trichloromethane and esterified with boratotrifluoricether/methanol. Heptadecanoic acid (17:0) was used as internal standard. The recovery rate of this method was 86.4% and coefficient of variation was 6.9%. The mean level of 25 normal adult serum samples was coincident with the data reported.
- 【文献出处】 营养学报 ,Acta Nutrimenta Sinica , 编辑部邮箱 ,1986年02期
- 【被引频次】29
- 【下载频次】349