【摘要】 用Leuconostoc mesenteroides 1226菌合成的右旋糖酐,作为血浆代用品已在临床普遍应用。据报道右旋糖酐的疗效和副作用与它的分子结构有关。本文介绍临床用右旋糖酐经衍生化制成各种部分甲基化乙酰化葡萄糖醇化合物之后采用气相色谱—质谱联用,测得右旋糖酐分子中主链、侧链连接位置;采用有效碳响应值计算右旋糖酐分子中各种糖苷键的平均重复单位并初步推断出右旋糖酐分子的结构式。更多还原

【Abstract】 Crude dextran, a fermentation product of cane sugar with Leuconostoc mesenteroides No. 1226, is partially depolymerized to obtain clinical dextran (D), which has been used effectively as blood volume expander. It has been. reported that side effects in clinical use is attributed to undesirable structure of D.The structure of D is determined by permethylation using Hakomori′s procedure, followed by acid hydrolysis, sodium borohydride reduction and acetylation. The methylated and acetylated components are then separated by gas chromatography and identified by mass spectroscopy. The results show that D contains α-D-(1→6) linkages in the main chain, with branched points of α-D-(1→3)and a few α-D-(1→2) linkages. The molar ratio of glucosyl residues with different linkages is calculated by using molar effective carbon response, and the structure of D is primarily postulated with an average repeating unit of 12 sugar residues.更多还原