【摘要】 本研究以人工配制的脱囊液在实验室条件下处理拉氏等孢球虫的孢子和卵囊,孢子在脱囊液胰酶+胆汁、胰酶+牛磺胆酸钠中均能脱囊。单独以胰酶、胆汁和牛磺胆酸钠培育的孢子则不能脱囊。胆汁类型(鸡、鸭、猪)对脱囊的速度和过程无影响。用上述脱囊液处理的卵囊不能脱囊。本文对子孢子的脱囊过程及其运动方式也进行了描述。更多还原

【Abstract】 In the present study, before treatment with trypsin, bile and sodium taurocholate, pretreatments of intact sporulated oocysts in a 50% CO2-50% air atmosphere for 5 hr or in 5.2% sodium hypochlorite for 40 min or in air alone were used. No excystation occurred when the oocysts were incubated in the following solutions; (1) . 0.5% trypsin and 8% chicken bile; (2) . 1.5% trypsin and 24% chicken bile; (3). 0.5% trypsin and 1.5% sodium taurocholate; and (4). 1.5% trypsin and 4.5% sodium taurocholate.Excystation occurred when sporocysts freed mechanically from oocysts were placed in excysting fluids,consisting of 0.5% trypsin and 8% bile (chicker.,duck and pig); 1.5% trypsin and 24% chicken bile; 0.5% trypsin and 1.5% sodium taurocholate? and 1.5% trypsin and 4.5% sodium taurocholate. Sporozoites failed to excyst when 0.5% and 1.5% trypsin were used without bile or when 8% and 24% chicken bile were used without trypsin. Sporozoites also failed to excyst when 1.5% and 4.5% sodium taurocholate were used without trypsin and bile. Pretreatment of sporocysts in a 50% CO2-50% air atmosphere before exposure to trypsin and bile or sodium taurocholate was not an essential for excystation of Sporozoites from sporocysts of I. lacazei,Excystation first observed within 3 to 11 min after the excysting fluid reached the sporocysts. The Sporozoites started moving, and the Stieda body became swollen and protruded, then it became indistinct and disappeared. The substiedal body began to protrude, appearing to push outward through the area earlier occupied by the Stieda body as if forced by pressure from within the sporocyst. Then the substiedal body suddenly popped out, leaving a opening through which the Sporozoites passed. Free Sporozoites were motile, exhibiting gliding and flexing movements.更多还原