【摘要】 对22例白血病、淋巴瘤病人和15例正常人进行了外周血淋巴细胞染色体脆性部位检测。结果表明,病人组的染色体畸变率、脆性部位检出率显著高于正常对照。通过G显带能准确定位的94个断点中包括了21种常染色体脆性部位,8个与癌基因在同一区带的断点。以上结果提示脆性部位同白血病、淋巴瘤之间有一定的相关。更多还原

【Abstract】 To determine the association of fragile sites with leukemias and lymphomas,we examined 22 patients with leukemia or lymphoma (6 Hodgkin lymphomas,6 non-Hodgkin lymphomas,5 acute lymphocytic leukemia,3 acute nonlymphocytic leukemia,2 lymphosarcoleukemia) and 15 normal controls ranging in age from 4 to 56 years.The results were: (1) The average chromosome structural aberration rate of patients(12.98%) was much higher than that of the control group(0.73%).Fifteen cancer patients carried fragile sites,12 of them carried multiple fragile sites,but none of the controls carried any.There was a statistically significant difference between the two groups (P<0.005).(2) These patients carried 21 autosomal fragile sites (including 14 constitutive fragile sites and 7 heritable fragile sites): 1q44,2q11,2q23,2q37,3p14,4q31,5q31,6q26,8q22,8q24,9q13,10q22,10q23,10q25,11q13,12q13,13q34,14q13,14q24,16p12 and 16q22.(3) Eight breakpoints were located at the bands where oncogenes exist: 1p31 and Blym-1,2p23 and Nmyc,6q23 and myb,8q22 and mos.8q24 and myc,11q13 and bcl-1,15q26 and fes,17q21 and erbA.(4) Four breakpoints were near the fragile sites: 1p32 near fra(1p31),2p23 near fra(2p24),7p15 near fra(7p14),7q22 near fra(7q21).One breakpoint(1p12) was near the Nras(lp13).Five of the fragile sites (8q22,11q13,12q13,16p12 and 16q22) correspond with cancer breakpoints.The role of fragile sites in carcinogenesis is not yet known,but according to our results the association of fragile sites with leukemias and lymphomas is evident which deserves further investigation.更多还原