【摘要】 采用附加植物激素的MS、N₆、百合、米勒和改良怀特培养基,培养平贝母单核中期的花药,诱导出一些愈伤组织（平均诱导频率为0.20％）,其中MS培养基的诱导效果最好。绝大多数再生植株出现在不加任何激素的1/2MS（其大量元素的含量是MS的一半）培养基上。再生植株的根尖细胞的染色体计数表明,约有1/4的细胞2n=12,证明再生植株是来源于花粉细胞的单倍体植株。在愈伤组织的分化培养中,建立起了能继代繁殖、不断保持绿苗分化能力的愈伤组织无性系。更多还原

【Abstract】 In anther culture of Fritillaria ussuriensis Maxim a number of calli were induced using MS,N?Baihe,Miller and were improved by White media supplemented with gome phytohormones.Average induction frequency of callus was 0.2%.MS medium had the best induction effect.Most of the regenerated plantlets appeared on 1/2 MS medium.Chromosome counting of root tip of the regenerated plantlets showed that the cells with 2n=12 made up a certain proportion and the plantlets were haploids derived from pollen.The domes have been established to keep successive propagation and ability of continual differentiation of green plantlets.更多还原