【摘要】 以胡麻叶斑病病原菌的毒素配制的培养基,培养水稻的体细胞愈伤组织,在其再分化第一代（R₁）植株群体中,发现3株(其中IR₅ 2株经25％毒素处理,IR₅₄ 1株未经毒素处理)抗胡麻叶斑病的突变。在这3个抗性系统的R₂（R₂植株的种子后代）群体中,均观察到对胡麻叶斑病病原菌的敏感与抗性的分离。本文是以植物毒素作为筛选因子,运用组织培养技术,在水稻上成功地筛选出抗病植株的首次报道。更多还原

【Abstract】 Two R1 plants of IR?and one of IR54 derived from 25% Helminthosporium oryzae toxin (H-tovin) treatment and toxin-free medium respectively were found to be resistant to the pathogen of brown spot disease.It was observed that resistant and susceptible plants were segregated in the R2 generation of R1 resistant plants.This is the first report of in vitro screening using phytotoxin for disease resistance in rice.In considering of the characteristics of Indica rice tissue culture i.e.low regeneration and rapid loss of regeneration capacity during subculture,a technique suitable for in vitro screening for disease resistance in Indica rice is established.The procedure consisted of using a very small pieces of callus (around 1 mm in size) and shaking in the toxin-enriched medium for 2 days and then transferring to an agar toxin-free medium for subculture and plant regeneration.The possible pathway of the mutation being established in vitro screening by phytotoxin was disccussed.更多还原