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激光微束照射切割PTK2细胞染色体的研究
Study on Dissection of Chromosome in PTK2 by Laser Microirradiation
【摘要】 把氬离子激光(5145)聚焦到PKK2细胞有丝分裂后期的染色体上,可以有选择性地诱发染色体的损伤而不影响细胞继续分裂直到形成两个子细胞。细胞化学分析证明,受激光照射的染色体区段的DNA遭到了损伤。讨论了激光显微外科术应用于染色体工程等遗传学研究的可能性。
【Abstract】 Chromosomal damage could be selectively induced and no deleterious effect occurred to continuous cell division up to the formation of two daughter cells,while argon laser (5145 A) was focused on mitotic chromosomes at anaphase in PTK,.Cytochemical analysis demonstrated that chromosomal DNA at the specific irradiated region was impaired.The possibility of laser microsurgery in genetics study such as chromosome engineering was discussed.
- 【文献出处】 遗传学报 ,Acta Genetica Sinica , 编辑部邮箱 ,1986年02期
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