【摘要】 应用凝胶电泳技术,对五个抗性不同的小麦品种苗期酯酶同工酶进行了测定.结果表明:小麦品种接种白粉病菌后,酯酶同工酶发生显著变化,相对酶活性随品种抗性下降而增强;不接菌时,品种间酯酶同工酶的差异甚微;幼苗不同器官和叶位的酯酶同工酶差异较大,相对酶活性依次是第三叶和假茎>第二叶>第一叶;接菌后,无病征叶片比有病征叶片的相对酶活性高.更多还原

【Abstract】 Esterase isoenzymes in wheat seedlings of 5 cultivars with different resistance to powder mildew(Erysiphe graminis DC.f.sp.tritici E.Marshal)were analysed by the polyacrylamide gel electrophoresis method.The results showed that,inoculated with E.graminis f.tritici,there were obvious differences among the electropherograms of esterase isoenzymes between the resistant and susceptible varieties;and the relative activities of esterase isoenrymes in the cultivars became stronger with the decrease of vatietal resistance to the powder mildew; but no marked difference was Observed among the esterase isoenzyme electropherograms of the same 5 cultivars which were not inoculated with the pathogenjthere were noticeable differences in the esterase isoenzyme alectropherograms of different organs and leaves on the same plant and the order of relative activities of esterase was in 3rd leaf and pseudostem >2nd leaf >lst leaf;after inoculation of seedlings with conidia of the pathogen, the relative activities of esterase isoenzymes in the symptomless leaves was stronger than in the symptomatic leaves.更多还原