【摘要】 用DEAE-纤维素（DE-22）并改进酶的提纯方法,聚丙烯酰胺凝胶电泳为单一蛋白区带的酶制剂比活力为359μM/mg（E）/min（Npp为底物）·用凝胶过滤法测得该酶分子量为58600,氨基酸组成有20种,共467个氨基酸残基。10^-4M pCMB使酶活力丧失98％;1×10^-3M PMSP该酶活力丧失96％,2×10^-4M NBS以及1×10^-2M BrAc酶活力均丧失90％·酶修饰失活呈一级反应,修饰过的酶紫外280nm吸收峰消失·初步判断文昌鱼AcPase分子上的Cys,Trp,His,Ser等氨基酸残基可能是酶的活力必需基团。更多还原

【Abstract】 Acid phosphatase of Amphioxus was purified by DEAE-cellulose DE-22 column chromatography. Specific activity of the enzyme preparation was359μM/mg(E)/min. The molecular weight was determined to be 58600 by gel filtration through Sephadex G-200, The enzyme molecule was found to beconstituted of 467 amino acid residues, among them 12 Cys, 6 His. 21 Ser, 4 Trp residues were observed.Inhibition of the enzyme with BrAc (1×10-2M) and PMSF(1×10-3M) in acid condition let to a rapid loss of activity. Enzyme activity appeared to be inhibited more than 90% by PCMB and NBS in the concentration of 1×10-4M and 2×10-4M respectively. The chemical modification studies of Acpase from Amphioxus exhibit that certain Cys, His, Trp and Ser residues are essential functional groups of this enzyme.更多还原