【摘要】 用909份甘蓝型油莱（Brassica napus L.）品种作试验材料,以花粉做媒介导入异种或同种有用基因,采用悬浮培养法,对花粉发芽进行了研究。结果:油菜花粉最适发芽的外界条件,是晴朗天气,气温25℃左右,午后1时取样,在温度25～30℃条件下发芽;花粉发芽最适的蔗糖浓度为30%;悬浮培养法,用通常的培养基（蔗糖30%,硼酸120ppm）进行发芽,最高萌发率仅在35%左右;普通培养基中附加2.4-D、NAA、IAA、GA₃各50ppm;或6-BA、KT、ZT 各50ppm;或2.4-D+GA₃、NAA+GA₃、IAA+GA₃、6-BA+GA₃、KT+GA₃、ZT+GA₃均为50ppm+30ppm 的不同生长激素处理,对花粉发芽均有显著促进作用。其中以细胞分裂素加 GA₃,促进发芽效果更佳,萌发率提高到85%以上;用2.5%果胶酶,1.5%RS 纤维素酶,以及30%蔗糖组成的酶液,成功地分离出油菜花粉原生质体。更多还原

【Abstract】 The germination of suspension culture pollens of Brassica napus L.cultivar 909 has been studied,by which we may use pollen as a tranmi-tter to introduce available gene.The main results are as follows:1.The most appropriate condition was that,in a fine day,atmospheric temperature is about 25℃,pollen were collected at 1∶00 p.m.andcultured at 20-25℃.2.The most proper concentration of saccharose for the germinationis 30%.3.Classic medium used for germination was adopted in suspensionculture（saccharose 30%,boric acid 120ppm）.The highest germinationratio was only about 35%.4.If the medium was supplemented with 2.4-D,NAA,IAA,GA₃ or6-BA,KT,ZT 50ppm respectively,or with 2.4-D+GA₃,NAA+GA₃,IAA+GA₃,6-BA+GA₃,KT+GA₃,ZT+GA₃ 50+30ppm respectively,thegermination would be promoted remarkably,especially the cytokinin plusGA₃,the germination ratio increased by more than 85%.5.Treat the pollens with the solution composed of 2.5% pectinase,1.5% cellulase（RS）and 30% saccharose,we isolated the pollen protoplas-ts successfully.更多还原