【摘要】 为鉴定中国人白细胞抗原(HLA)系统,本文采用微量淋巴细胞毒试验方法,筛选武汉地区非孕经产妇血清1000份,获得淋巴细胞毒反应强度20％以上的阳性血清136份,其中70％以上16份,总阳性率13.6％,强阳性率1.6％。136份阳性血清中98份阳性血清进行了相关集群,共集得16组显著相关的淋巴细胞毒抗血清,希望通过集群获得HLA分型血清。本文对筛选结果和试验方法进行了讨论。更多还原

【Abstract】 To achieve identification of HLA in Chinese,the screening of lymphocytoto-xic antisera in 1000 nonpregnant multiparous women was carried out in Wuhan,Hubei Province.We adopted the standard microlymphocytotoxic techniques in this study.136 cases (13.6% of all screening sera) were positive with lymphocytoto-xic activity.Sixteen (1.6%) of positive sera were of strong lymphocytotoxic activity.Ninety eight of 136 positive sera were retested against panel cells of 80 donors which were not HLA-typed.Subsequently,the 98 positive sera underwent grouping by which sixteen groups were obtained.The calculation of correlation coefficients and the analysis of grouping were performed by a computer.The results and methods of screening are discussed in this paper.更多还原