【摘要】 以间接免疫荧光法检测用热溶猪卵透明带抗原(HSPZ)和分离的猪卵透明带抗原(IPZA)免疫家兔与恒河猴所获得的抗血清,结果显示:兔抗HSPZ 血清的阳性滴度是1:3800,兔和恒河猴抗IPZA 的血清的阳性滴度分别是1:3400和1:1800。经猪肝粉及猪固相血浆吸收后,抗HSPZ 血清的滴度明显下降:但抗IPZA 血清的滴度仅轻度下降。HSPZ 及IPZA 均具有较好的免疫原性,然而HSPZ 含有较多的非特异性物质。实验结果还表明:猪卵透明带抗原和鱼卵透明带抗原之间仅有部分交叉。更多还原

【Abstract】 By using indirect immunofluorescence test we studied the actions of antisera raisedfrom rabbit and rhesus monkey with heat-solubilized porcine zona pellucida(HSPZ)and isola-ted porcine zona pellucida(IPZA)as antigens.Results showed that the positive titer of an-ti-HSPZ in rabbit was 1:3800 and the positive titers of anti-IPZA were 1:3400 and 1:1800in rabbit and monkey respectively.After absorption with porcine liver powder and solid plasma,the titer of anti-HSPZwas significantly declined,while that of the anti-IPZA was declined only slightly.Asantigens,both HSPZ and IPZA possessed high immunogenicity,but HSPZ contained morenonspecific substances.The experimental results also showed that the antigens of HSPZ and fish ova hadpartial cross-reaction only.更多还原