【摘要】 利用杂交瘤技术制备了11株抗人绒毛膜促性腺激素（hCG）的单克隆抗体。根据这些抗体和¹²⁵I 标记的hCG,hCGα,hCGβ和hLH 结合的情况,可将抗体分为三类。抗体BAH₂作用于hCG_α和β亚单位结合后所暴露出的抗原决定簇,抗体10E₅和8D₅特异地和hCGβ亚单位结合但不和hLH 结合。大多数抗体是作用于hCG 和hLHβ亚单位上共有的决定簇。实验显示这些单克隆抗体特异性优于多克隆抗体,它们亲合常数在5.3×10₇～7.2×10¹⁰M^-1之间。更多还原

【Abstract】 Eleven strains of anti-hCG monoclonal antibodies were prepared.These antibodieswere tested for binding with ¹²⁵I labelled hCG,hCGα,hCGβ and hLH.Antibody BAH₂acted against an epitope which was exposed only after α and β subunits combination.Most ofthe antibodies could bind with hCG,hCGβ or hLH.This suggests that these antibodieswere against the common epitopes of hCGβand hLHβ.Antibodies 10E₅ and 8D₅ only boundwith the specific epitopes of hCGβ.The affinity constants of these antibodies were between5.3×10⁷M^-1～7.2×10¹⁰M^-1:更多还原