【摘要】 用杂交瘤技术获得了第二组抗人血小板的单克隆抗体。这些单克隆抗体在体外对正常人血小板聚集功能有不同程度和性质的影响。其中SZ-21的E亚克隆分泌的单克隆抗体能完全抑制胶原诱导的血小板聚集并能部分抑制肾上腺素、ADP诱导的血小板聚集。SZ-21E单克隆抗体影响血小板功能的性质,提示其识别的抗原决定簇可能是血小板膜糖蛋白Ⅱ/Ⅲ。其他各株细胞及其亚克隆也有进一步研究的价值。更多还原

【Abstract】 We have obtained the second group of monoclonal antibodies against human platelet by means of hybridoma technique. These antibodies can in vitro influence the normal platelet aggregation at different degrees and types. Among them, the monoclonal antbody of the “E” subclone from SZ-21 cell line can entirely inhibil the platelet aggregation induced by collagen and partly inhibit the aggregation in-duced by adrenaline and ADP. It is suggested that the epitope identified by SZ-21E antibody be platelet membrane glycoprotein Ⅱ/Ⅲ judging from the charac-teristic impact on platelet function. Nevertheless, the other cell lines and their subclones are worthy of being further studied.更多还原