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~3H-TdR掺入法测定NHG-1脾淋巴细胞转化功能
The Transformation of NHG-1 Spleen Lymphocyte Was Determined by Using the ~3H-TdR up Taken Method
【摘要】 本文报告用~3H-TdR掺入法测定本室的NC系正常裸小鼠和荷人脑胶质瘤裸小鼠(NHG-1)的脾淋巴细胞转化功能。结果提示:正常的纯合子(nu/nu)T细胞缺乏,B细胞功能均正常;杂合子(nu/+)的T和B细胞功能均正常;荷瘤鼠(NHG-1,30代)除了T细胞缺乏,B细胞功能亦受到抑制。
【Abstract】 In this report, the transformation of normal nude mice and NHG-1 spleen lymphocytes were determined by using the 3H-TdR up taken method. The results showed that the transformation of T and B cells were normal in heterogametic mice(nu/+)While it was lacking of T cells and nomal B cell transfomation in homogenotic mice(nu/nu), In tumour carried mice(NHG-1, through 30 generations) there were not only lack of T cells, but also restrai-ned on the transformation of B cells.
【关键词】 裸小鼠;
同位素掺入法;
脾淋巴细胞转化;
【Key words】 nude mice; up taken method of isotope; transformation spleen lymphocyte;
【Key words】 nude mice; up taken method of isotope; transformation spleen lymphocyte;
- 【文献出处】 苏州医学院学报 ,Suzhou University Journal of Medical Science , 编辑部邮箱 ,1986年04期
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