【摘要】 本文以大叶黄杨茎段外植体作材料,研究了自然脱落和乙烯、ABA诱导叶柄脱落过程中过氧化物酶活性和同工酶谱的变化。自然脱落的叶柄在陈化时过氧化物酶活性逐渐增加,到72小时达最高值。乙烯和ABA在加速脱落的同时提高了酶活。用CHI处理减弱了上述两种激素脱落效应的同时降低了酶活。离区吲哚乙酸氧化酶的酶活与过氧化物酶呈正相关性。自然脱落与ABA处理的叶柄离区,其过氧化物酶同工酶有新谱带出现,而经乙烯处理过的却没有明显的谱带变化。文章就激素与有关酶类的控制与被控制的关系从而调节脱落的观点进行了讨论。更多还原

【Abstract】 Peroxidase activity in the abscission zone of explant of Euonymus japoncia Thumb. increases graduelly in the process of natural abscission. For normally growally growing abscission zone, two major bands were observed on its peroxidase isozyme, and four new bands appeared after 72hrs of natural abscission. Peroxidase activity in abscission zone and abscission of explant increased after treatment of 10ppm ethylene or 10ppm ABA. Both the accelerated abscission and peroxidase activity enhanced by ethylene or ABA was inhibited by cycloheximide. It is proved that peroxidase is synthesised de nove during the course of induced abscission. The explant treated by ethylene or ABA has a differently changed peroxidase isozyme. After 48hrs of ABA treatment, three new bonds appeared, while no new band was observed after ethylene treatment. ABA stimulated ethylene production and that is one of the possible reason that ABA promoted abscission.更多还原