【摘要】 用耳蜗灌流和电生理技术,研究了L—天冬氨酸（L—ASP）和草酰乙酸（Oxa）对豚鼠耳蜗电位的影响。对照灌流液为人工外淋巴液（Elliotts’液）,试验液分别为20或25mM的L—ASP或10mM的Oxa,均做鼓阶灌流以进行比较。灌流液由电子微量泵驱动。在灌流过程中记录由短声引起的CM和APN₁,并在微处理机上作常规处理。20mM的L—ASP使APN₁振幅降低49％,P<O.01。25mM的L—ASP使APN₁振幅降低36％,在L—ASP的两种浓度时CM均无变化。1OmM的Oxa对APN₁和CM均无明显影响。L—ASP在豚鼠耳蜗内似为强去极化因子。高浓度时使突触后膜强去极化。L—ASP可能对Ca²⁺起作用, 间接影响递质的释放。更多还原

【Abstract】 The effects of L-Aspartate and Oxaloacetic acid on cochlear potentials of guinea pig were investigated with perfusin and electrophysiological technique. The control solution were artificial perilymph solution(Iliott’ s solution)and the experimental solution were the 20mM L-Aspartate ,25mM L-Aspertate, or 10mM Oxaloacetic acid respectively. All the experimental solution were perfused through the scala tympani of guinea pig in order to compare with control solution. The perfusion solution was pushed by electrical micropump. The click-evoked CM and APN, were recorded during these perfusion and controlled routines with microcomputer(KDS-I).In these tests perfusions with 20 mM L - Aspartate produced depression of APNI 49% , P<0.01.In these tests perfusions with 25 mM L-Aspartate also decreased the amplititude of APNI 36%.The Both concentrations 20 mM L-Asp and 25mM L - Asp without effect on CM.The experiments perfusions with lOmM Oxaloacetic acid had no effect on APNI and CM.The effect of L-Aspartate in cochlea of guinea pig appear to be a potent depolarising agent,and the effect of high concentration of it on postsynaptic membrane was higher degree of depolarization.It is supposed that the L-Aspar-ate may be to cause the change of Calcium and indirectly effect on the release of transmitter.更多还原